The Contractile Response of Lung Pericytes to Reactive Oxygen Metabolites
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Authors
Kerkar, Sid P.
Issue Date
1999
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Regulation of pericyte contractility by reactive oxygen metabolites (ROM) may
play a significant role in contributing to the regulation of microvascular fluid
hemodynamics during inflammation, sepsis, and the adult respiratory distress syndrome
(ARDS). ROM, which are known mediators in the pathophysiological response of
sepsis, have been shown to induce both relaxation and contraction in large blood vessels.
The purpose of this study was to test the effects of ROM on lung pericyte contractility
and whether these effects could be attenuated by antioxidants. Cultured pericytes were
exposed to hydrogen peroxide and pyrogallol at 10 µM, 100 µM, and 1 mM, while the
antioxidant effects of catalase (100 µM), super oxide dismutase (SOD; 100 µM), and
vitamin E pretreatments (1 mM) were also quantified. Both hydrogen peroxide and
pyrogallol induced dose-dependent relaxation at 10 minutes. However, at 30 minutes
dose-dependent contraction was measured. Catalase completely attenuated both
physiological responses, while SOD did not have an effect on either. Vitamin E,
however, only partially attenuated contractions induced at 30 minutes but had no effect
on the relaxation at 10 minutes. These results suggest that ROMs are capable of
inducing both relaxation and contraction in lung pericytes, implying two separate time
dependent pathways by which contractility is regulated.
Description
iv, 25 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.