Effects of Interleukin-1 on the T Lymphocyte Subset Distribution in Arthritic and Normal MurineLymphoid Tissues

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Authors
Zielkowski, Natalie
Issue Date
1989
Type
Thesis
Language
en_US
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Abstract
Collagen-induced arthritis (CIA) in mice is used as a model for human rheumatoid arthritis (RA). Interleukin-1 is a protein hormone that mediates many biological activities associated with inflammation and is hypothesized to play a role in the immunologic and inflammatory processes of both RA and CIA. In the present study, the influence of in vivo administration of IL-1 on the T lymphocyte subset distribution in the lymphoid tissues of collagen-arthritic and normal mice was assessed. Mice were immunized intradermally with chicken type II collagen. On day 18 after immunization, normal and immunized mice were injected once daily for three days with recombinant human IL-1β The thymus, spleen, popliteal lymph node and inguinal lymph node were removed before and one day after IL-1 treatment. Immunohistochemical analysis of the cells revealed a decrease in the number of T lymphocytes, mainly the T suppressor subset, in the lymph nodes and an increase in the T helper cell population in the thymus of post IL-1 treated mice. T cell populations decreased slightly in the IL-1 treated spleens. These results indicate that IL-1 has an effect on the T cell distribution in murine lymphoid tissues. The significance of these findings to the immune response.
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vi, 49 p.
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Kalamazoo College
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U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
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