Time Stability of the Binding Affinity of 5-Hydroxytryptophan to DFHBI- 1T- Broccoli RNA Complex Using Fluorescence Detection
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Authors
Neville, Alaya M.
Issue Date
2019-09-01
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Aptamers are an alternative to antibodies with a longer shelf life, multi-diverse target capabilities, effective production rate, and thermal flexibility. Our focus was to explore the optimal conditions and methods for our DFHBI-1T RNA biosensors to bind to the serotonin precursor 5-hydroxytryptophan (5-HTP) in vitro that can mimic in vivo conditions. We tracked our fluorescent markers and determined their binding affinities via fluorescence-based assays. Through a series of 15-minute interval timed assays set at standard conditions we found that when measured at 25Æ C, the binding affinity of biosensors CM 2, CM 5, CM 6, CM 7 and CM 8 did not change over a 45- minute time period, while biosensors CM 3 and CM 4 did change. Conversely, we found that when measured at 37Æ C, biosensors CM 3, CM 4 and CM 5 change over a 45- minute period; CM 2 and CM 5- CM 8 did not.
Description
vii, 20 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.