The Induction of Apoptosis in Macrophages with Activated Human Cytolytic T 4 Cell Clones
Loading...
Authors
Buckmaster, Tarek R.
Issue Date
1992
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Macrophages, activated through cytolytic T cell recognition of their
presented antigens, undergo cell death in the maintenance of homeostatic
control over cell numbers. We examined the interaction of activated human
T4 cell clones with macrophages to determine if helper T-Iymphocytes could
be induced into initiating the cytolysis of macrophages. We sought to
compare cytolytic capabilities of different T-Iymphocyte cell lines through
their ability to express a cytoplasmic trypsin-like granular enzyme, serine
esterase. The cloned T4 cells and the cytolytic T8 cells contained similar levels
of serine esterase activity, while freshly isolated T4 cells showed negligible
activity. Thus, cloned T4 cells become activated during culture. A previous
report indicated that murine T cells became activated between the seventh
and ninth days of culture, although the means is not yet known.(Lancki et al.
1991) We attempted to determine if the length of activation for cultured
human T4 cells was equivalent to the murine cells, but the esterase activity
levels did not reach significant amounts during the nine days of the test
culture. When cultured T4 cells were combined with antigen-presenting
macrophages and stimulated by the tetanus toxoid antigen, isolated DNA
showed signs of fragmentation through gel electrophoresis. These
fragmented segments of nuclear DNA, approximately 200 base pairs in length,
displayed that apoptosis was occurring within the macrophages.(Wyllie 1980)
Thus, cultured human T4 cells express cytolytic serine esterase and can
initiate the apoptotic mechanism within antigen-presenting macrophages.
Description
iii, 27 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.