Visualizing and Quantifying the Vascularity of Mouse Bone via Immunohistochemistry
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Authors
Wright, Robert N.
Issue Date
2020-04-01
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Bone formation, bone metabolism, and the remodeling process is believed to be highly dependent on the vascular network within bone. The structural and spatial relationship between the vascular network and bone, however, is not very well known nor studied specifically within the cortical bone. Thus, this study focuses on implementing a consistent and accurate immunohistochemistry protocol that quantifies and shows the complexities of the vascular network within the cortical bone vasculature within the long bone of mice. This study was not able to implement a consistent and accurate immunohistochemistry protocol that quantifies and shows the complexities of the vascular network within the cortical bone vasculature within the long bone of mice. Thus, India ink stained cortical bone vasculature was visualized and quantified within the long bone of mice. It was determined that 8% of all vascular pores were India Ink stained within the cortical bone of the long bone of mice. Furthermore, radiopaque contrast solutions were explored in order to further develop the structural and spatial relationship between the vascular network within the cortical bone of the long bone of mice. These radiopaque solutions are injected into the mouse right ventricle and imaged using computed tomography capable of reaching nominal resolutions as high as 1 μm. Solutions consisting of barium sulfate and gelatin suspended in 1x PBS were identified as possible radiopaque solutions along with future directions are explored within this paper.
Description
vii, 29 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.