Replication of Tanapox Virus in Owl .Monkey Kidney Cells and Human Glioblastoma-Astrocytoma Cells
Sket, Georgina M.
Tanapox virus (TPV) is a human and primate pathogen that causes relatively mild, self-limiting febrile illness. It is a member of the genus Yatapoxviridae in the poxvirus family. The virus only replicates in human and primate cells. It is speculated that TPV infection is spread through a biting arthropod vector. Green fluorescent protein (GFP) is a chemoluminescent protein that can be incorporated into virus DNA and serve as a marker for infection. A recombinant version of Tanapox virus (TPVGFP) is frequently used in studies involving TPV. This study aims to examine whether the incorporation of the GFP gene into the TPV DNA negatively affects virus replication rate within ~ts host. Furthermore, this study will observe replication rates at 35°C and at 37°C in order to corroborate claims that the virus also grows at temperatures lower than primate physiological temperature. It is hypothesized that, in keeping with previous claims, temperature will not affect the virus replication rate. It is also hypothesized that there will not be a difference in replication rates between TPV-CDC and TPV-GFP. In this experiment, TPV-GFP and TPV-CDC wer~ grown in Owl Monkey Kidney (OMK) cells and Human Glioblastoma-Astrocytoma (U373) cells. A virus titer was then performed using OMK cells. Using ANOVA there was found to be significant variation within the means, and Student's t-test found the differences between the means ofTPV-CDC grown in OMK cells at 37°C and TPVCDC grown in U373 cells at 35°C to be significantly different from other means at the p > 0.05 level. Therefore, while the hypothesis that temperature does not affect replication was proven, our study claims that the inclusion of GFP into the viral genome negatively affects the overall replication rate of the virus.
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