Analysis of Drug Resistance Patterns in 13762NF Rat Mammary Adenocarcinoma Cell Lines
Loading...
Authors
Hathaway, Karen M.
Issue Date
1988
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
To study the effects of cellular subpopulation interactions on
metastatic stability in polyclonal populations of 13762NF rat
mammary adenocarcinoma cell lines, the restriction of clonal
diversity was required. This clonal restriction was achieved
through drug exposure and selection of drug resistant cell lines
(Adr-, DAP-, Oua-, TG- and Vcr-resistant) to act as cell markers.
The objective of this research was to identify the patterns of drug
resistance of these selected MTF7(T35).3 parent and drug-resistant
cell lines using clonogenic colony formation assays to assess
survival after 7 d of continuous drug exposure. Dose-response
curves were constructed from average percent survival data, 90%
lethal dose (LD90) levels were calculated from these dose-response
curves, and levels of drug resistance were determined from these
values. All cell lines were resistant to their respective selecting
agents except the Qua resistant cell line, but the level of resistance
varied when compared to the parental F35.3 cell line. Crossresistance
was observed primarily for the Vincristine sulfate
resistant cell line but to a lesser degree for other cell lines.
Individual resistance patterns of the cell lines did not change in coculture
indicating no cooperativity between cell lines. These cell
lines will be useful for future experiments designed to study the
stability of metastatic potential.
Description
v, 33 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.