The Variation of Invasion of Metastatic Cancer Cell Lines Across Sea Urchin Basement Membranes Over the Cell Cycle
Loading...
Authors
Rossing, Matthew J.
Issue Date
1996
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Metastasis is the most deadly aspect of cancer, leading to the spread of tumors
throughout the body. An important facet of cancer is invasion across basement
membranes. In this paper, we look at the variation of invasion frequency over the cell
cycle using basement membranes from embryos of the sea urchin Strongylocentrotous
purpuratus as a model of invasion. This model has previously been shown to give good
correlation with metastatic potential in vivo. The drug lovastatin is used to synchronize
the metastatic cancer cell lines, which are then placed on basement membranes at varying
parts of the cell cycle. Invasion frequencies are assayed microscopically. Flow cytometric
analysis using propidium iodide DNA staining in conjunction with anti-bromodeoxyuridine
staining is then used to determine in which phases of the cell cycle invasion is high or low.
In the three cell lines tested, there is an apparent variation of invasiveness over the
cell cycle. Preliminary data suggest that cells are most highly invasive during S phase.
Future work will confirm this or determine exactly when in their growth cycle cancer cells
are most invasive. Differences between phases with high invasion frequencies and low
invasion frequencies should help direct further research into the mechanisms of invasion of
metastatic cancer cells.
With honors.
With honors.
Description
v, 30 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.