An Analysis of the Antimicrobial Properties of Melatonin-Coumarin Hybrids
Antibiotics have helped to treat, destroy, or slow down the growth of bacteria causing diseases. Unfortunately, through the process of natural selection antibiotic-resistant strains of bacteria have been allowed to live and reproduce, rendering many antibiotics ineffective. That is why in modern medicine it is paramount that new, innovative antibiotics are constantly being developed. Multiple literatures have exhibited antibacterial activity of coumarin and melatonin. Thus, the idea of hybridizing melatonin-coumarin species via synthesis was proposed. A previous study was conducted to synthesize the melatonin-coumarin hybrid compounds where they were tested for their antimicrobial activity via disc assay. Due to the lack of solubility of the hybrids and lack of controls the results for that study were inconclusive. In this study, it was investigated whether the hybridization of coumarin and melatonin compounds would increase the potency of their individual antibacterial affects against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus cereus through 96-well plate experiments. Controls for these hybrid compounds were then synthesized and purified confirmed with NMR. Low to moderate percent yields for these control compounds were found. The hybrids that were synthesized in a previous lab, as well as, the controls synthesized in this study were tested against the same bacteria to determine if the compounds possess antimicrobial activity. It was concluded that none of these compounds demonstrated antimicrobial activity due to the increase of Optical Density 600 values after 24 hours of incubation found via plate reader.
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