Traumatic Brain Injury Causes Increased Gut Permeability by Attenuation of the Tight Junction Proteins Zonula Occludens Protein-l and Occludin

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dc.contributor.advisorBansal, Vishal
dc.contributor.advisorSotherland, Paul R., 1953-
dc.contributor.authorKroll, Lauren M.
dc.date.accessioned2011-12-05T16:16:36Z
dc.date.available2011-12-05T16:16:36Z
dc.date.issued2009
dc.descriptionvi, 23 p.en_US
dc.description.abstractMillions of individuals sustain traumatic brain injury (TBI) each year in the United States. In many cases of TBI, the body responds with a cascade of progressive illnesses, including systemic inflammatory response syndrome (SIRS), multisystem organ failure (MSOF), and eventual death. SIRS contributes greatly to MSOF by promoting sepsis through the breakdown and increased permeability of the intestinal epithelium, normally maintained by the tight junction proteins zonula occludens-l (ZO-l) and occludin. We hypothesized that TBI would initiate an increase in intestinal permeability by attenuation of ZO-l and occludin. We used a weight drop model to successfully initiate moderate TBI in mice. We confirmed the effectiveness and accuracy of the model by neurological evaluation as well as by histological (brain) procurement and evaluation. We investigated effects of induced TBI on intestinal epithelium by histological (intestinal) procurement and evaluation. Intestinal histology .of animals experiencing TBI demonstrated both blunting and abnormally large spacing of the intestinal villi, indicating breakdown of the intestinal epithelium following TBI. By both an in vivo intestinal permeability assay and Western blotting, we found that, in TBI animals, intestinal permeability increased while intestinal ZO-l and occludin concentrations decreased when compared to sham animals. In conclusion, we propose increased intestinal permeability following TBI to be secondary to the attenuation of tight junction proteins ZO-l and occludin. Ours is one of the first studies to demonstrate this finding, which has extensive clinical applications for individuals experiencing TBI.en_US
dc.description.sponsorshipDepartment of Surgery. University of California. San Diego, California.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10920/24204
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleTraumatic Brain Injury Causes Increased Gut Permeability by Attenuation of the Tight Junction Proteins Zonula Occludens Protein-l and Occludinen_US
dc.typeThesisen_US
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