The In Vitro Effects of Luteinizing Hormone on Rat Ovarian Mitochondria
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Authors
Labbe, Stephen D.
Issue Date
1975
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Mitochondria were isolated from the ovaries of mature
rats. Polarographic techniques were used to measure state
III resp1ration, State IV respiration, Respiratory Control
Ratios (RCR) ,and ADP:O ratios. Addition of Luteinizing
Hormone (LH,5O ug) in vitro had no effects on any parameter
of ovarian or liver (control) mitochondrial respiration.
The effects of 3',5'-Adenosine Monophosphate (c-AMP)
and Adenosine Monophosphate (AMP) on ovarian mitochondrial
respiration were also studied. Cyclic AMP (0.3 mM) caused
significant increases in state III (p<0.00l) and state IV
(p<0.0l) respiration, while not affecting RGR or ADP:O
ratios. Liver mitochondria, used as a control, were not affected.
AMP (0.3 mM) had similar effects as c-AMP.
Various methods of isolating ovarian mitochondria were
attempted in order to maximize mitochondrial yields while
minimizing losses in viability.
Description
vi, 33 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.