Genetic Variation among Individuals of Batrachospermum helminthosum from Spring Brook, Kalamazoo, Michigan
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Authors
Filkin, Nanda R.
Issue Date
1998
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
A study was conducted which examined genetic relatedness among 21 individuals
of Batrachospermum helminthosum from Spring Brook, a stream in Kalamazoo,
Michigan. The molecular marker technique, ISSR (Inter-Simple Sequence Repeats), was
employed. This technique is new and has been used only once before to understand
variation in algae. ISSR is a form of PCR (Polymerase Chain Reaction) in which DNA is
replicated between specific micro satellite loci. These specific loci are determined by the
primers used. In this study, six ISSR primers were used- ISSR 4, 8, 9, 10, 11 and 12.
Gel electrophoresis was used to separate the replicated portions of DNA into bands.
Bands were scored as 1 if present and 0 if not for each individual. This raw data (1 's and
O's) was examined using PHYLIP (Phylogeny Inference Package) and a UPGMA tree
showing relative relatedness among individuals was created. Relative relatedness within
the stream was explained by controlled gene flow within mini populations. Overall, little
gene flow occurred and within this population, minipopulations with related genomes
were maintained within small clusters.
Description
vi, 21 p.
Citation
Publisher
Kalamazoo College
License
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