Synthetic Cellular Scaffolds with Biomimetic Properties Interact Favorably with Osteoblastic Cells
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Authors
Pack, Sean
Issue Date
2005
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
There has been a push to discover new synthetic polymers with biomimetic
properties that support the proliferation and migration of osteoblastlbone cells. By
fabricating polymers into cellular scaffolds that interact with cells on the molecular level
this field is moving closer to growing transplantable bone. The benefits of
transplantation of bone would have profound effects on orthopaedic and maxillofacial
surgical procedures. In this study, we used the murine MC3T3-EI (subclone 26)
osteoblast cell line to seed pre-fabricated scaffolds. We employed scaffolds fabricated
using two techniques: the first was Solid Free Form (SFF) fabricated, and the second was paraffin sphere fabricated. Both the SFF and paraffin sphere fabricated scaffolds were made from the same synthetic polymeric material, poly-L-lactic acid (PLLA). Using the SFF technique we fabricated solid/control and nano-fibrous PLLA scaffolds. Using the paraffin sphere technique we fabricated solid/control, nano-fibrous, and surface modified nano-fibrous (SMN) PLLA scaffolds. The SMN fibrous scaffolds had a three-molecular thick layer of gelatin coating the exterior of an otherwise normal nano-fibrous scaffold. This paper is based on results from three experiments. The first was a growth
experiment that investigated the difference in cellular growth, migration, penetration, and
collagen secretion of solid, nano-fibrous, and SMN paraffin sphere PLLA scaffolds. The
second experiment investigated the growth, migration, penetration, and collagen secretion of cells seeded on SFF fabricated solid and nano-fibrous PLLA scaffolds. In both the paraffin sphere and SFF fabrication growth experiments DNA assay did not support the expected differences in growth over two weeks; however, with regard to both scaffold types, histological analysis did support the expected penetration patterns. In the third experiment we used SFF fabricated solid and nano-fibrous scaffolds to provide data that would enable future experimenters to optimize growth conditions for osteoblasts. This was a six-week differentiation experiment that used three media combinations and compared cell growth, migration, and penetration. The histological data from this experiment supported the hypothesis that a combination of the media components would likely best suit the differentiation of osteoblast cells.
Description
viii, 35 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.