Intercellular Adhesion Molecule-2 (ICAM-2) Expression in the Mouse Eye
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Authors
Solem, Amanda
Issue Date
1999
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Intercellular adhesion molecule-2 (ICAM-2) is an important factor in leukocyte
recirculation and trafficking (Nortamo et ai., 1991; Staunton et ai., 1989). Recent studies
(Xu et ai., 1996; Hobden et ai., 1999) have suggested that ICAM-2 may function as an
intercellular adhesion molecule-l (ICAM-1) surrogate, which recruits polymorphonuclear
leukocytes (PMN) during an inflammatory response. The purpose of this study was to
identify ICAM-2 expression in the murine eye and to compare it to that of ICAM-1.
ICAM-2 and ICAM-1 expression were studied in both an outbred (Swiss) and an inbred
strain (129/Sv) of mice. The ICAMs were detected by immunofluorescence (ICAM-1
and ICAM-2) and by an immunohistochemical technique (ICAM-2) on frozen sections of
mouse eyes. To study ICAM-2 and ICAM-1 expression during an inflammatory
response, mouse eyes were infected with Pseudomonas aeruginosa for 6 hr and then
sectioned. ICAM-2 was constitutively expressed on vascular endothelium in the
conjunctiva and the anterior iris. It was not upregulated following P. aeruginosa
infection. ICAM-1 was expressed on vascular endothelium in the conjunctiva and on
several other cell types in the eye constitutively. During infection, ICAM-1 was
upregulated and expressed on conjunctival stromal fibroblasts and vascular endothelium,
corneal epithelium, corneal stromal keratocytes, and corneal endothelium. Previous
reports ofICAM-1 expression in the eye (Figdor et ai., 1992; Foets et ai., 1993;Goldberg
et ai., 1994; Hobden et ai., 1999; Vadas et ai., 1992; Vorkauf et a/., 1993) and reports of
ICAM-2 expression on vascular endothelium were confirmed (Xu et a/., 1992; Xu et ai.,
1996; de Fougerolles et ai., 1991). ICAM-2 and ICAM-1 are both found on vascular
endothelium in the eye, supporting the hypothesis that ICAM-2 is an ICAM-1 surrogate.
Description
iv, 23 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.