Attenuation of Mutation in the Mitochondrial Genome Through Supplementation of DNA Polymerase y Will Slow Aging-Related Decline of Function
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Authors
Horowitz, Justin
Issue Date
2004
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Gradual decline of function can be attributed to the accumulation of mutations in mitochondrial DNA. These mutations arise due to imperfect proofreading by DNA polymerase y, a family A DNA polymerase and the only known mitochondrial exonuclease and replicase. As evidence exists for interchangeability of family A polymerases, this proposal suggests a method for confirming the possibility of interchanging or supplementing DNA polymerase y with E. coli DNA polymerase I. While both enzymes have exonucleolytic activity in the 3'->5' direction, E. coli DNA polymerase y contains an additional 5'->3' exonuclease domain that enhances its proofreading capacity. If the hypothesis is successfully upheld, it suggests a course of gene therapy that may be able to delay the onset at-symptoms of advanced aging in humans.
Description
iv, 20 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.