The Effects of Nerve Growth Factor on the Growth and Survival of the A875 Human Melanoma Cell Line
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Authors
Burns, Aime
Issue Date
1989
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Nerve growth factor (NGF) is a classical example of a
protein that promotes survival, neurite extension, and
transmitter production in neurons. It also affects other
types of cells, some of which are neural crest-derived. One
of these cell lines, A875 , a human melonoma cell line, was
examined for changes in growth and survival in response to
the addition of purified mouse NGF to culture medium. When
A875 cells are exposed to 2.5S NGF (50 ng/ml) in culture
media that are slightly permissive (0.25, 0.50, 0.75%
serum), proliferation and growth are enhanced. In contrast
to cells grown without NGF, when cells were exposed to NGF
in these serum concentrations, cell proliferation was
stimulated at a faster rate. These effects were less
pronounced in medium depleted of serum growth factors or
medium that is highly permissive (> 1% serum). These
findings show that NGF has the capacity to increase
proliferative activity of the cells and may act as a
survival factor. They also show that sufficient FBS
concentration is needed for survival of the melanoma line
and that some serum factor is needed for the activation of
NGF. The present results demonstrate that murine NGF
produces biological activity in the A875 cell line. The
effects of NGF on growth appear analogous to its actions on
the development of the sensory and sympathetic neurons and
other neural crest-derived cells.
Description
v, 21 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.