Studies on Tumor Growth Inhibition by Anti-Angiogenic Drugs as Studied on Walker 256 Rat Carcinoma Tumors Grown on the Chorioallantoic Membrane of the Chick Embryo
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Authors
Burkart, Thomas A.
Issue Date
1989
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
An assay for the effect of experimental angiostatic drugs (drugs
found to inhibit neovascularization) on growing tumors was
developed. Walker 256 rat carcinoma cells were grown in culture
and implanted on the chorioallantoic membranes (CAM's) of seven
day old chicken embryos. Initial tumor growth and survival studies
indicated day seventeen to be the best day to weigh drug treated
tumors due to the substantial size of the tumor at this time and the
sufficiently high survival rate of the tumor-bearing embryos on this
day. Growing tumors were treated on day 8 or 10 of embryo
incubation with the angiostatic drugs L-azetidine (LACA),
tetrahydrocortisone (THS) and Upjohn's U42129 with and without
heparin or Beta-cyclodextrin tetradecasulfate. Embryos were
sacrificed on day 17 of incubation, their tumors removed from the
CAM and weighed. Although photographic results of certain drug
treatments showed a trend in that drug treatment to affect tumor
growth, one way analysis of variance of all drug treatment data
showed none of the treatments to significantly affect tumor growth.
Further studies involving multiple drug treatments, varying drug
dosages and how best to apply the drugs as well as other parameters
of this assay remain to be done before a conclusion can be drawn as
to the effectiveness of this assay in the testing of experimental
angiostatic drugs.
Description
vi, 40 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.