Properties of Breast Epithelial Cancer Cell Line after Treatment with Modified Citrus Pectin and the Transfection of Non-galectin-3 Expressing Cell Line with Cleaved-Galectin-3 cDNA
This study aims to expand the work done with modified citrus pectin (MCP) by using a new line of breast preneoplastic epithelial cells, EIII8s. The unique capability of this cell line included its ability to form ductal tissue when injected into nude mice and that they express an estrogen receptor not common to other breast cancer cell lines. Previously, mice were injected with EIII8 cells and tumor growth occurred. One set of mice was being fed MCP, and after a month their tumors began to shrink. The tumors were isolated and grown in the lab in EIII8 MCP and Control. Additionally, we attempted to create an expression vector for cleaved-galectin-3, a glycorecognition protein that is responsible for a number a metastasis properties. A number of methods were used for testing the effects of MCP on EIII8 cells. Boyden chambers, growth in hypoxia conditions, a soft agar experiment and growth rate analysis were used to determine the effects on the cells after in vivo treatment with MCP. Standard DNA manipulation techniques were used to create a new expression vector of cleaved-galectin-3 in PCN-C10. These were then transfected into BT-549, which do not. normally express galectin-3 to determine if the expression vectors were viable. Results from the EIII8 study showed very little differences between the cell lines. It was also found that MCP does not affect galectin-3 levels in either cell line when under hypoxia conditions. Attempts to express cleaved galectin-3 failed, but may have been due to problems with cell lysate samples.
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