Construction and Characterization of a Plasmid Borne Prokaryotic-Eukaryotic Fusion RNA Polymerase Largest Subunit
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Authors
Rieden, Cynthia L.
Issue Date
1991
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
A novel plasmid, pKC91, was created. The plasmid encodes the
Escherichia coli RNA polymerase largest subunit β rpoC gene with an
altered carboxyl-terminal region. The C-terminal region was modified
to include the heptapeptide repeat of Saccharomyces cerevisiae RNA
polymerase largest subunit. The repeat has the consensus sequence
Tyr-Ser-Pro-Thr-Ser-Pro-Ser, and has been implicated in the processes
of DNA binding and RNA chain elongation. The addition of this
region was found to have no effect on growth rate or protein
expression of transfected cells. It did interfere with functional
complementation in rpoC deficient strains. The addition of the
heptapeptide repeat disrupts function of the produced RNA
polymerase largest subunit, potentially by steric hinderance of the
catalytic site, inhibition of the ability to complex with other subunits to
form the holoenzyme, or by replacement of a region essential for
function.
Description
iv, 33 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.