A Spectrum Analysis of Hepatitis B Virus Strain and Drug Bound State Variances
Authors
Major, Samantha
Issue Date
2023-11-01
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The purpose of this research was to perform an icosahedral point array spectrum analysis on the two strains and five CPAM drug bound states of the hepatitis B virus (HBV) capsids to determine possible key structural points of the viral capsid. This project serves to further the research towards the prevention and disruption of HBV infection. This research has been done using ViperDB coordinates of each structure, VMD to visualize differences between structures and unique features of the HBV capsids, Matlab to analyze point array data for each structure and determine the fitness of each point array, and analysis using code created by Gabe Orosan-Weine (K’23) to determine the closest amino acids to each point in each point array. This data was all analyzed for each structure and compared to determine differences that may be caused by each drug and similarities that may indicate necessary features of the viral capsid. These methods produced data that suggests that point arrays 3, 4, 6, 20, 52, 53, and 54 have a high fitness for most, if not all, structures studied, meaning that the points in these arrays likely fall near key structural features of the capsid. It also showed that certain amino acids are repeatedly close to said points, implicating a probable importance to chain and capsid stability. These results provide potential targets for engineered mutations, structural changes, or other changes to the viral capsid that can disrupt or prevent viral infection of HBV.
Description
71 p.
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License
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