Protective Effects of Grape Seed Proanthocyanidin Extract IH636 against Ischemia and Reperfusion Injury in HL-I Cardiomyocytes
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Authors
Engers, Drew W.
Issue Date
2005
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Given the current prevalence of cardiovascular disease, seeking forms of
protection against myocardial injury is of significant importance. Grape seed extract
poses a potential solution to this dilemma since it is a potent antioxidant which could
combat the damaging effects of reactive oxygen species (ROS) and electrophiles in
cardiomyocytes. However, other than its capacity to act as a free radical scavenger, the
mechanistic function and effects of grape seed in cardiomyocytes to promote an antioxidant response are not fully understood. The aim of this study was to investigate in
vitro cardiac myocytes subjected to the intense oxidative stress of ischemia/reperfusion in order to delve into the possibility of differing mechanisms of benefit conferred by
variations in treatment time and dose. Thus, HL-l cardiomyocytes were either acutely
(30 min) or chronically (24 hr) treated with IH636 grape seed proanthocyanidin extract
(GSPE) in a dosage range of25-100 J.lg/ml prior to ischemia/reperfusion (I/R). The
levels of necrosis and apoptosis were measured with lactate dehydrogenase (LDH)
release and DNA fragmentation assays with ELISA, respectively, giving insight into
protection against certain cell death pathways. Supplementation with 100 J.lg/ml of GSPE produced marginally significant reductions in necrosis levels of both acutely and
chronically treated cells and in the apoptosis levels of chronically treated cells. This
study suggests that a 100 µg/ml GSPE dose may be a physiological significant treatment concentration for in vitro cardioprotection of HL-l cells. More replicates of this research are necessary to determine the true statistical significance of this treatment concentration as a protective measure against necrotic and apoptotic cell death for cardiomyocytes.
subj ected to IIR.
Description
vi, 30 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.