Two Dimensional Electrophoretic Analysis of the Relative Turnover Rates of Individual Subfornical Organ Proteins under Conditions of Water Deprivation and Salt Loading
Santer, Donna Marie
Fluid balance is important for all living organisms, and the subfornical organ of the brain, a midline, circumventricular organ, has a well established has a role in the regulation of fluid balance in higher animals. For four days, three groups of rats were given an appropriate fluid diet (control, 2% NaCl, and water deprived), with all groups having free access to food. Rats were killed by decapitation and the SFO quickly dissected out, and incubated in medium containing 35S-methionine and 35S-cysteine for 6 hours. The proteins from these organs were then separated by two-dimensional electrophoresis. The gels were then stained, photographed, dried and exposed to autofluorographic film. The results show that relative turnover rate of 8 proteins were changed due to experimental manipulations.
iii, 24 p.
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