Two Dimensional Electrophoretic Analysis of the Relative Turnover Rates of Individual Subfornical Organ Proteins under Conditions of Water Deprivation and Salt Loading
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Authors
Santer, Donna Marie
Issue Date
1985
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Fluid balance is important for all living organisms, and the
subfornical organ of the brain, a midline, circumventricular organ, has
a well established has a role in the regulation of fluid balance in
higher animals. For four days, three groups of rats were given an
appropriate fluid diet (control, 2% NaCl, and water deprived), with all
groups having free access to food. Rats were killed by decapitation and
the SFO quickly dissected out, and incubated in medium containing
35S-methionine and 35S-cysteine for 6 hours. The proteins from these
organs were then separated by two-dimensional electrophoresis. The gels
were then stained, photographed, dried and exposed to autofluorographic
film. The results show that relative turnover rate of 8 proteins were
changed due to experimental manipulations.
Description
iii, 24 p.
Citation
Publisher
Kalamazoo College
License
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