In vitro effects of (-)-epigaIl0-3-catechin gallate on induced inflammation response in human gingival fibroblasts and EpiGingival™ tissue models
Bianchi, Erik M.
Periodontal diseases constitute a major concern in oral healthcare. Periodontitis and gingivitis, together, present a problem twice as large as tooth decay. These diseases are characterized mainly by inflammation, but also by the destruction of the connective tissue that supports the teeth in the oral cavity. Anti-inflammatory drugs have been developed to counteract tissue inflammation, but long-term use of these drugs may lead to severe side effects. For this reason, research is being aimed towards finding natural agents that can be used to suppress inflammation and manage inflammatory diseases. Previous evidence suggested that (-)-epigallo-3-catechin gallate (EGCG) may suppress inflammation in gingival cells and that green tea could be used as a therapeutic agent in maintaining and managing periodontal diseases. The present in-vitro study seeks to detennine the effects of the green tea catechin, EGCG, on induced inflammation in human gingival fibroblasts (HGF) and EpiGingival™ tissue models. Both human gingival fibroblasts and EpiGingival Thf tissue models were used to model true oral tissue; model inflammation was induced by TNF -α. and was measured using an ELISA technique. The results of the study did not provide a solid conclusion that EGCG had a strong effect on TNF-α induced secretion of IL-8, a pro-inflammatory protein, in either cell model. The study did, however, show a synergistic effect of EGCG at low concentrations, which opens up this research for further study. Data analysis was conducted using SPSS software.
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