Cloning, purification and characterization of B cell Activating Factor
In animals, the antibody repertoire of B cells plays an important role in immune protection. In rabbits, immune B cells develop in the bone marrow and migrate to the gut associated lymphoid tissues (GALT), where, in the presence of commensal bacteria, they are stimulated to proliferate, form organized follicles and develop the primary antibody repertoire by Ig gene diversification. While the molecular events leading to Ig gene diversification in the bone marrow are well understood, little is known about the mechanisms regulating B cell proliferation and homeostasis in GALT. Possible mechanisms are shown in Figure 1. In a previous study to test whether B cell activating factor (BAFF) may play a role in stimulating GALT B cells, endogenous BAFF was neutralized in vivo using a soluble decoy receptor. This treatment greatly reduced the number and size of proliferating B cell follicles, demonstrating that BAFF is required for B cell development in GALT. However, it was not clear from this study whether BAFF provided a proliferation signal or survival signal. Therefore to address this question, I cloned, expressed and purified recombinant rabbit BAFF from E. coli, and tested its affect on B cells in vitro
Kalamazoo, Mich. : Kalamazoo College.
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