Analysis of the KHRI-3 Antigen in Guinea Pig Inner Ear and Lung Tissues
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Authors
Braunz, Andrea E.
Issue Date
2004
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Autoimmune Sensorineural Hearing Loss (AISNHL) is a quickly progressing
hearing loss that results from an immune response in one's body. In an effort to further
understand and more effectively treat AISNHL, a monoclonal antibody (KHRI-3) and a
polyclonal antibody (rabbit anti-CTL2) were developed, using the antigen found in
AISNHL patients, for research using guinea pigs as a model. When the KHRI-3 antigen
is probed with the KHRI-3 or the rabbit anti-CTL2 antibody after immunoprecipitation
and SDS-PAGE, a doublet of approximately 68 kDa and 72 kDa is seen in Western Blots.
Probing homogenates of guinea pig organs (brain, tongue, colon, and lung) with either
antibody resulted in a single band with a molecular weight of -70 kDa To investigate
why the inner ear extracts appear as a doublet, we performed several experiments dealing with the glycosylation on the KHRI-3 antigen. We hypothesized that sialic acid residues accounted for the upper band of the doublet in the inner ear antigen. However, the experiments demonstrated that sialic acid residues are found on the KHRI-3 antigen
collected from the inner ear as well as the lung, revealing that the upper band in the inner ear is not due to sialic acid residues. Further research is needed to determine why the inner ear extracts appear as a doublet.
Description
v, 25 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.