Preliminary Characterizations of Tendon and Spleen Isolates from Chickens Infected with Persistent Avian Reovirus Strain, 1733
Loading...
Authors
Palileo, Ruth Pe
Issue Date
1992
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The avian reovirus strain, 1733, is known to persistently infect gastrocnemius
tendons of chickens, causing arthritis. Chickens less than a day old or 1 day
old were inoculated with 1733. Viral isolates were obtained from the spleen
and the tendon of these chickens at 7, 14, 21 and 49 days post-infection. Chick
embryo fibroblasts (CEF) infected with both spleen and tendon isolates
evidenced a syncytia-like clumping of cells prior to lysis, behavior not
normally observed in the parental strain. This behavior was more marked in
CEFs infected with spleen isolates. However, tendon cells infected in the
same way evidenced this behavior to a greater extent when infected with
tendon isolates. Phenotypic observations were made using
immunoflourescent antibody assays. Overall, isolates obtained at a longer
time post-infection showed a greater degree of syncytia-like behavior.
Preliminary genotypic characterizations were made using polyacrylamide gel
electrophoresis for RNA; gels were subsequently silver-stained. Some small
migrational shifts occurring in the L2 segment of certain isolates indicate
genetic alteration. This data suggests that such syncytia-like clumping of cells
prior to lysis results from persistence of strain 1733 in either or both tendon
or spleen cells.
Description
iii, 17 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.