Effect of Polymorphonuclear Cells on Invasive and Metastatic Potentials of 13762NF Rat Mammary Adenocarcinoma Cells
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Authors
Schissel, Daniel John
Issue Date
1988
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The number of circulating activated polymorphonuclear
leukocytes (PMNs) increases in tumor-bearing rats in a manner
corresponding to the metastatic propensity of the tumor cell
inoculated. Because secretion of basement membrane degrading proteinases
was also enhanced, it was proposed that activated PMNs may
play a role in tumor metastasis, particularly tumor cell
extravasation. To test this hypothesis, we measured the
effects of normal PMNs and circulating PMNs isolated from
peripheral blood of tumor bearing rats on the experimental
metastatic and invasive potentials of 13762NF rat mammary
adenocarcinoma cell clones. Circulating PMNs isolated from
MTLn3 tumor-bearing rats increased the invasion potentials of
13762NF rat mammary adenocarcinoma clones in vitro when tested
in the Membrane Invasion Culture System (MICS) using
reconstituted basement membrane as a barrier, and experimental
metastatic potentials in vivo in a dose dependent manner. In
contrast, 'normal' PMNs isolated from peripheral blood, or
proteose peptone-stimulated peritoneal exudate did not alter
these malignant characteristics. When rats received
intravenous co-injections of tumor-elicited PMNs and tumor
cells at an effector:target (E:T) ratio of 50:1 the mean
number of experimental lung metastases rose 23. 2-fold for
weakly metastatic MTLn2, 1.6-fold for moderately metastatic,
highly invasive MTF7 clone, and 3.0-fold for the highly
metastatic MTLn3 clone. In vitro invasive potentials were
measured in MICS with varying E:T ratios (up to 30:1) of
tumor-elicited PMNs and tumor cells. Invasive potential
increased up to 25.5-fold for MTLn2, 36.7-fold for MTF7, and
53.7-fold for MTLn3. These results indicate that PMNs
isolated from MTLn3 tumor-bearing rats have properties that
can contribute to the metastatic potentials of other 13762NF
rat mammary adenocarcinoma clones by assisting their invasion
through a reconstituted basement membrane.
Description
vii, 34 p.
Citation
Publisher
Kalamazoo College
License
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