The Effects of the Selective Kappa Antagonist Nor-Binaltorphimine on Dopaminergic Systems of the Rat Brain and Posterior Pituitary

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dc.contributor.advisorLookingland, Keith
dc.contributor.advisorManzanares, Jorge
dc.contributor.authorLa Vigne, Suzette D.
dc.descriptionvi, 42 p.en_US
dc.description.abstractThe specific effects of a selective kappa opioid-receptor antagonist nor-binaltorphimine (nor-bni) on the activity of nigrostriatal, mesolimbic, tuberoinfundibular and tuberohypophysial dopamine (DA) neurons were examined in the rat brain and posterior pituitary. The ability of U50488, a selective kappa opioid agonist, to reverse the effects of nor-bni was also investigated in order to determine if nor-bni was acting specifically at a kappa receptor. DA neuronal activity was estimated by measuring: (1) DA synthesis (dihydroxyphenylalanine (DOPA) accumulation after the administration of an aromatic amino acid decarboxylase inhibitor), (2) DA metabolism (dihydroxyphenylacetic acid (DOPAC) concentrations), and (3) plasma levels of prolactin and a-MSH, hormones know to be regulated by tuberoinfundibular and tuberohypophysial DA neurons , respectively. Nei ther U50488 or nor-bni had any effect on the activity of nigrostriatal or mesolimbic DA neurons, indicating that these neurons are not regulated by kappa receptors. Tuberoinfundibular DA neurons were activated by nor-bni, but this effect was not reversed by U50488 and therefore represents a non-specific action of nor-bni. Tuberohypophysial DA neurons are also activated by nor-bni, and this effect was reversed by U50488, revealing a kappa receptor-mediated tonic inhibition of tuberohypophysial DA neurons.en_US
dc.description.abstractWith honors.
dc.description.sponsorshipDepartment of Pharmacology and Toxicology. Michigan State University. East Lansing, Michigan.
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Biology Senior Individualized Projects Collection
dc.relation.ispartofseriesSenior Individualized Projects. Biology;
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.
dc.titleThe Effects of the Selective Kappa Antagonist Nor-Binaltorphimine on Dopaminergic Systems of the Rat Brain and Posterior Pituitaryen_US