The Search for a Metastasis Suppressor Gene for Prostate Cancer on Human Chromosome 17
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Authors
de la Paz, Michael G.
Issue Date
1996
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The enigma of prostate cancer is determining which of the many cases of prostate cancer
diagnosed will acquire metastatic ability. For this reason, effort is underway to locate
metastasis suppressor genes for prostate cancer. In previous studies, Rinker-Schaeffer et al. (1994) used microcell transfer to fuse human chromosome 17 with AT6.1, a highly metastatic R-3327 dunning rat cell line, and discovered a novel metastasis suppressor
region. In this SIP, the polymerase chain reaction was performed on the DNA of the
metastatic revertants of four metastasis suppressing clones to determine if nonrandom
deletions had occurred. Results show a nonrandom loss of the sequence tagged site
D17S791 which suggests that it is the locus of the metastasis suppressor region. More
detailed molecular mapping of this region in the future may provide an exact location of
the metastasis suppressor gene. In addition this paper shows the results of the subcloning
sequencing and BLAST search results performed using the products of differential display
(which compares expressed DNA) previously performed on AT61.1 cells and the
metastasis suppressed microcell hybrids, cell line AT6.1-17-4. BLAST searches revealed
that several of the sequences had high identities to known cDNA sequences with unknown
functions, and on the sequences appears to be a novel gene. These sequences are
candidate genes for metastasis suppressor function because their functions are unknown.
One sequence is a human homolog of the fission yeast cell cycle regulator gene nuc2 and
two other are sequences with high identities to importin which is a homologue of the
Drosophila tumor suppressor oho61. All of these sequences should be considered
candidate genes responsible for metastasis suppression in human prostate cancer.
Description
vi, 31 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.