A Novel Mutagenesis Strategy to Inactivate the Gene Encoding IgAl Protease in Neisseria gonorrhoeae for Use in the' Human Challenge Model
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Authors
Koymen, Hakan O.
Issue Date
1994
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Immunoglobulin Al (IgAI) protease is believed to be a possible virulence
factor of Neisseria gonorrhoeae. However, until this point it has been impossible to
study the role of IgAl protease in vivo. To avoid the addition of any new antibiotic
resistance markers, our laboratory had developed and refined a novel two step
mutagenesis strategy which allows us to manipulate a specific gene. Through this
method we have successfully constructed an isogenic mutant which is unable to
produce a functional IgAI protease. Furthermore, we have characterized our mutant,
and found that all traits, including lipooligosaccharide, pilin type, and opacity proteins,
remain intact and unchanged. Since we have not affected the genome in any other
way, and have not given the bacteria any antibiotic advantages, we are now prepared
to test the mutant by experimental urethral infection in our human challenge model.
Description
v, 43 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.