Analysis of Human Chromosome 18q in Squamous Cell Carcinoma of the Head and Neck
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Authors
Welch, Joshua A.
Issue Date
1997
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Chromosome segment loss within the long arm of chromosome 18 (18q) is a frequently
observed aberration in many advanced cancers, including squamous cell carcinomas of the head and neck (SCCHN). Loss of heterozygosity (LOH) often indicates that the region of loss marks the locus of a deleted or altered gene, which in its normal state functions to suppress tumor growth. Therefore, the mutation of one allele and loss of the other leads to cancerous cellular proliferation. Three potential tumor suppressor genes on 18q have been associated with other forms of cancer. In order to determine if any of these play a role in SCCHN, we examined normal, tumor, and tumor cell line DNA from SCCHN patients by Microsatellite Sequence Repeat Polymorphism (MSRP) analysis. Using DNA primer sets at polymorPhic markers spanning 18q, we amplified specific regions of simple sequence nucleotide repeats by polymerase chain reaction (PCR). We tested centromeric (UMI-3) and telomeric (MBP) markers, as well as several somewhat evenly spaced markers between them, with DNA from 11 previously studied patients as well as 11 new patients. We were able to begin the process of
defining precise areas of common loss, although no conclusive links to potential tumor
suppressor genes can currently be established. Our results support previous research that links 18q loss with tumor progression.
Description
vi, 21 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.