Analysis of Human Chromosome 18q in Squamous Cell Carcinoma of the Head and Neck
Welch, Joshua A.
Chromosome segment loss within the long arm of chromosome 18 (18q) is a frequently observed aberration in many advanced cancers, including squamous cell carcinomas of the head and neck (SCCHN). Loss of heterozygosity (LOH) often indicates that the region of loss marks the locus of a deleted or altered gene, which in its normal state functions to suppress tumor growth. Therefore, the mutation of one allele and loss of the other leads to cancerous cellular proliferation. Three potential tumor suppressor genes on 18q have been associated with other forms of cancer. In order to determine if any of these play a role in SCCHN, we examined normal, tumor, and tumor cell line DNA from SCCHN patients by Microsatellite Sequence Repeat Polymorphism (MSRP) analysis. Using DNA primer sets at polymorPhic markers spanning 18q, we amplified specific regions of simple sequence nucleotide repeats by polymerase chain reaction (PCR). We tested centromeric (UMI-3) and telomeric (MBP) markers, as well as several somewhat evenly spaced markers between them, with DNA from 11 previously studied patients as well as 11 new patients. We were able to begin the process of defining precise areas of common loss, although no conclusive links to potential tumor suppressor genes can currently be established. Our results support previous research that links 18q loss with tumor progression.
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