Characterization of the cDNA and Gene for the Heart/Muscle Isoform of Cytochrome C Oxidase Subunit VIII
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Authors
Brown, Jessica Sutherland
Issue Date
1995
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
The mouse heart cDNA library in Lambda ZAP ll was screened for the
heart/muscle isoform of cytochrome c oxidase (COX) subunit VIII. Two
clones were isolated and sequence data showed a 100% nucleotide sequence
identity that encoded a protein 70 amino acids long with a 24 amino acid
presequence and a 46 amino acid mature protein. Comparisons of the mouse
cDNA sequence with the rat and bovine cDNAs helped to identify the C-terminal
hydrophilic region as exhibiting the greatest conservation. A stretch
of 6 completely conserved amino acids was identified that could later be used
to prepare degenerate probes for evolutionary comparisons among primates.
Humans have been shown to lack the heart isoform of COX subunit VIII and
this study may help identify when in evolutionary history the gene was lost.
The 5' flanking region of the mouse COX8 heart gene was also sequenced and
proximal promoter and enhancer elements were discovered by comparing
conserved sequences with known bovine enhancer elements. The
transcription start site in the bovine sequence was highly conserved with a
region in the mouse sequence though the start site has not yet been mapped
in the mouse. A TATAA box and two E-box elements, one on either side of
the TATAA box, were identified in the mouse gene that were conserved with
sequences in the bovine promoter demonstrated to increase transcription in
myotubes. Therefore, these elements may play a role in tissue specific
expression of the gene in skeletal muscle in mice. Moreover, a potential
CArG box or NRF-2 site was also identified in the 5' untranslated region of
the gene that may also regulate expression.
With honors.
With honors.
Description
v, 37 p.
Citation
Publisher
Kalamazoo College
License
U.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder.