Growth Curve Analysis and the Influence of the Chemopreventive Agent Curcumin on the Bacteria Prevotella Melaninogenica and Capnocytophaga Gingivalis from the Oral Cavity
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Authors
Drake, Daniel R.
Issue Date
2004
Type
Thesis
Language
en_US
Keywords
Alternative Title
Abstract
Metabolites of Helicobacter pylori, Salmonella typhi and Neisseria spp. have
been implicated in gastric, gall bladder, and colon cancer through specific metabolic
interactions. Preliminary results from a recent study showed that Capnocytophaga
gingivalis and Prevotella melaninogenica were associated with oral cancer lesions, acting as diagnostic indicators for Oral Squamous Cell Carcinoma (OSCC). The aim of this project was to elucidate the growth curve for P. melaninogenica and C. gingivalis for
reference in future studies analyzing their characteristic metabolisms. C. gingivalis
cultures were inoculated into an enriched liquid broth supplemented with L-cystein and
glucose and the absorbence was taken of the concentration over a time of 72 hours. P.
melaninogenica was inoculated into trytpic-soy broth supplemented with vitamin-K and
the absorbence was measured over 48 hours. The exponential phase for P.
melaninogenica and C. gingivalis was achieved approximately 6 and 12 hours,
respectively, following inoculation. In order elucidate the effects of anti-cancer treatment
on the two bacteria, curcumin, a polyphenol with antimicrobial properties, was tested
against the bacteria to find the Minimum Bactericidal Concentration (MBC). Curcumin
was dissolved in dimethylsulfoxide and inoculated into enriched agar media for testing
against the bacteria. Separate cultures of P. melaninogenica and C. gingivalis were
plated on agar containing the curcumin dilution standards. In vitro, P. melaninogenica
remained resistant to all concentrations of curcumin tested. Curcumin effectively .
inhibited C. gingivalis at a MBC of 64µg/ml. Oral species may play a major role in the
development of oral cancer, specifically OSCC, by metabolizing certain carcinogens and
these studies may shed light on the uses of chemopreventative agents.
Description
v, 22 p.
Citation
Publisher
Kalamazoo College
License
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