Comparison of Rat a-GST ELISA to Rat a-GST Activity Assay for Use in High Throughput Toxicity Screening

Abstract

In this investigation, four compounds were chosen to test an assay that would be easier and less expensive to run. Although this experiment was set up to compare results of the Rat a-GST ELISA and the GST Activity lines, the biological markers MTT, ATP, and cell number were included to show similar responses occurred given the treatment the cells received. Overall, with the trends seen in the results, a definite strong correlation exists between the two assays.