Synthesis of OligodeoxyriboDucleotides on Cellulose Filter Discs using the Phosphoramidite Triester Coupling Approach

Abstract

DNA is a very stable molecule involved in the storage of genetic information. It is a polyester chain of nucleotides, generally double-stranded with a helical conformation. The DNA chain is made up of three main components: a heterocyclic base a carbohydrate moiety and a phosphate bridge as illustrated in figure 1. The heterocyclic bases are either a pyrimidine, thymine (1) or cytosine (2), or a purine, adenine (3) or guanine (4). The carbohydrate moiety is a 2'-O-deoxy-Dribose in a furanose form. The bases are attached at the N-9 of purine bases or N-1 of pyrimidine bases to Col of each sugar through a beta-linkage to form a unit called a nucleoside. The sugar units joined through a phosphate bridge at their C-3 and C-5 hydroxyl groups to form a 3',5'linkage are called nucleotides.