The Effect of Lectins on Murine Embryonic Stem Cell Differentiation
Wearne, Kimberly A.
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Lectins are carbohydrate-binding proteins shown to be important in the hemagglutination of cells and blood typing. The effect of three lectins on the differentiation of Embryonic Stem (ES) Cells was determined. Two of the lectins were purified from the mushroom Marasmius oreades (MOA) and the plant Amaranthus caudatus (Amaranthin). The third I purified from the mushroom Polyporus squamosus (PSL). Previous work has suggested that the lectin MOA enhances the formation of endothelial cells from ES cells, based on increased expression of the endothelial cell-specific marker Flkl. Murine D3 ES cells were differentiated by embryoid body formation in the absence and presence of lectin and the mRNA levels were examined using quantitative Reverse Transcriptase Polymerase Chain Reaction. Our results indicate that although endothelial cells formed from the differentiating ES cells, the addition of lectin did not enhance their formation. However, the MOA and PSL lectins may enhance endothelial cell tube formation.