Quantitating Gene Expression in Murine Liver Using Microarray Technology
Microarrays are useful in studying gene expression on a large scale. By printing eDNA of interest onto a chip and performing hybridization, the levels of gene expression from different organisms can be compared. In this study, our lab examined the impact of dioxin, phenobarbitol, and cobalt chloride treatment, as well a knockout ofAhr on gene expression in liver tissue ofmice at different stages ofdevelopment. The results from various PCRs are shown, as well as the printing results from a sample V4 chip. Because the actual V4 chip was not ready to be printed, data from another chip is shown to demonstrate the methods ofhybridization and analysis ofmicroarrays.. The data from this chip will be used in the continuing goal ofthe Bradfield lab to identify genes that are expressed in response to the carcinogen dioxin, and other polycyclic aromatic hydrocarbons. It will also be used in detecting when certain PAS superfamily genes responding to those polycyclic aromatic hydrocarbons are expressed during different stages of development.