Proteomic Evaluation of a Putative Saccharomyces Cerevisiae IRE Knockout

Abstract

Proteomics is developing into one of the most effective methods to analyze the biology of an organism, eventually leading to the determination of function. After establishing a successful proteomics procedure, this technology was implemented in the evaluation of an IRE Knockout (KO) involving the Unfolded Protein Response in Saccharomyces cerevisiae. 2D-PAGE and subsequent analysis of proteins by the MALDI-Tofmass spectrometer identified proteins in yeast which were previously published in a yeast 2D gel database. This demonstrated that proteomics protein identification technology was successfully completed. However, no proteins were identified that provided evidence of an IRE KO. Subsequent to these experiments researchers providing the KO samples completed molecular biology experiments, which conferred that an IRE KO was not successfully established.