Development of a Method for the Chiral Seperation of a Pharmaceutical Compound by Capillary Electrophoresis
Vasas, Jennifer M.
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High-performance capillary electrophoresis using chiral selectors has been shown to be a useful analytical method in determining the drug purity of enantiomers in the pharmaceutical industry. In our model system, an uncoated fused silica capillary was used with a background electrolyte of pH 2.5. Heptakis-6-sulfato-~-cyclodextrin (HS-~- CD) was used as the chiral selector for the separation of the enantiomers PNU-142774 and PHA-513550. The results are presented regarding the effect of operational variables, such as buffer concentrations, analytes, and pH, capillary temperature, applied electric field, and concentration and type of chiral selectors. Using the experimental data, the operational parameters were optimized to attain maximum resolving power.