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dc.contributor.advisorFenno, J. Christopher
dc.contributor.authorHollandsworth, Anne M.
dc.description1 broadside : ill.
dc.description.abstractPeriodontal Disease • Periodontal disease is a bacterial infection of the gums and supporting structures in the mouth, including ligament and alveolar bone, which is triggered by the build-up of microorganisms that create a biofilm known as plaque (Bascones-Martinez and Figuero-Rutz, 2004; Liebana et al., 2004). Treponema denticola • Treponema denticola is a Gram-negative, anaerobic, motile and helical rod which belongs to a group of bacteria known as the “red complex” that is frequently associated with periodontitis (Edwards et al., 2003; Kuramitsu, 2003). • Numerical prevalence, high association level and spatial location within infected sites suggest T. denticola plays a key role in disease progression via a number of virulence factors (Fenno and McBride, 1998). Purpose • The purpose of this investigation was to disrupt two potentially virulent genes in T. denticola: TDE1343 and TDE 1658, using a gene transfer system developed by Li and Kuramitsu (1996). • TDE1343 is a homolog of the htrA/degP gene in E. coli which codes for a heat-shock protein. The HtrA/DegP protein combines the activities of the chaperones and the proteases to control the degradation of misfolded proteins (Strauch and Beckwith, 1988). • TDE1658 is a homolog of the surA gene in E. coli which codes for a periplasmic peptidyl-prolyl isomerase. SurA was found to be involved in the correct folding and maturation of outer membrane proteins thus enabling the cell to communicate with the external environment (Behrens et al., 2001).en
dc.description.sponsorshipDepartment of Biologic and Materials Sciences. School of Dentistry. University of Michigan. Ann Arbor, Michigan.
dc.description.tableofcontentsIntroduction -- Materials and methods -- Results -- Discussion -- Literature cited -- Acknowledgments
dc.publisherKalamazoo College
dc.subject.lcshGums -- Diseases
dc.subject.lcshTeeth -- Care and hygiene
dc.titleTargeted gene disruption by homologous recombination in the oral spirochete Treponema denticola ATCC 35405en

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  • Diebold Symposium Posters and Schedules [479]
    Poster and oral presentations by senior biology majors that include the results of their Senior Integrated Projects (SIPs) at the Diebold Symposium. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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