Activity-Dependent Phosphorylation of GLUR1/4 by PKA is Subunit Specific
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Authors
Wilson, Christopher G.
Issue Date
2002
Type
Presentation
Language
en_US
Keywords
Alternative Title
Abstract
α-amino-3-hydroxy-5-methyl-4-isoxazole
propionate (AMPA) receptors are ionotropic glutamate
receptors that mediate fast excitatory responses in
neurons. Receptors containing subunits GluR1 or
GluR4 are involved in the initial response to synaptic
activity, and are known to be phosphorylated by
protein kinase A (PKA) at serine residue 845 and 842,
respectively. Through several experiments employing
Western Blot analysis of rat hippocampal slices using
phospho-specific antibodies, we have been able to
determine how spontaneous synaptic activity affects
this phosphorylation. The results show that synaptic
activity does lead to the phosphorylation of GluR4 by
PKA but does not regulate GluR1 phosphorylation.
Additional results show that the number of
phosphorylated GluR1 and 4 subunits increases with
age. It is then suspected that although the cell may
use these subunits for similar purposes, the
biochemical regulation of their delivery to synapses
may be different.
Description
1 broadside : ill.
Diebold Scholar
Diebold Scholar
Citation
Publisher
Kalamazoo College