|dc.description.abstract||Small nuclear ribonucleoparticles (snRNPs) are a class of protein-RNA complexes found in the
nucleus of eukaryotic cells. snRNPs are essential particles involved in the post-transcriptional
processing of RNA. A subclass of snRNPs, the small nucleolar ribonucleoparticles (snoRNPs), are
localized to the nucleolus and are involved in processing of the rRNA precursor transcript (prerRNA)
and ribosome biogenesis. The U3 snoRNP is an essential snoRNP involved in the cleavage
and methylation of pre-rRNA. These events are critical for the proper assembly of the ribosome.
Four proteins, Nop1p, Nop56p, Nop58p and Snu13p have been shown to bind to the U3 snoRNA,
and form the U3 core complex. Because large protein complexes isolated from archaeal sources
seem to be more amenable to crystallization than those from eukaryotic sources, it may be useful to
study the structure and function of the archaeal U3 snoRNP. While no U3 complex has been
observed in Archaea, proteins homologous to many U3 proteins do exist in Archaea. We have
amplified genes encoding the U3 proteins Nop1, Nop56/58 from two archaeal genomes, S.
solfataricus and M. jannaschii, as well as a Snu13 homolog from M. jannaschii. These genes have
been cloned into prokaryotic expression vectors and expressed in E. coli cells. Recombinant Nop1p,
Nop56/58p and Snu13p proteins will be purified by heat treatment and column chromatography.
They will then be assayed for complex formation and for assembly upon the U3 snoRNA. These
complexes will be purified and used in crystal trials for the determination of the crystal structure.
This structure will provide some insights into the role of the U3 complex in pre-rRNA processing
and will provide the basis for a structural and functional comparison of rRNA processing between
Archaea and Eukarya.||en