JavaScript is disabled for your browser. Some features of this site may not work without it.
  • About K
  • Academics
  • Admission
  • Alumni Relations
  • Giving to K
  • News & Events
  • Student Life
  • HORNET HIVE
  • ATHLETICS
  • SITEMAP
  • WEBMAIL
    • Login
    View Item 
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Diebold Symposium Posters and Schedules
    • View Item
    •   CACHE Homepage
    • Academic Departments, Programs, and SIPs
    • Biology
    • Diebold Symposium Posters and Schedules
    • View Item

    Function of the TAD/PEST Domain of the Mouse Notch1 and Notch4 Genes

    Thumbnail
    View/Open
    poster presentation (143.7Kb)
    Date
    2002
    Author
    Flewelling, Daniel T.
    Metadata
    Show full item record
    Abstract
    The Notch gene is highly conserved and found in most, if not all metazoans. The primary function of Notch proteins is to determine cellular fate during embryogenesis, but Notch plays a vital role in adult organisms as well. Improper Notch signaling has been linked to neoplasia, stroke and possibly schizophrenia in adult mammals. Figure 1 illustrates proper Notch signaling. Multiple Notch homologs exist in worms, zebrafish, chickens, mice and humans. The exact functions of the multiple Notch genes has yet to be determined. Mouse Notch proteins are the most similar to human Notch proteins, and for the purpose of this study, mouse Notch1 and Notch4 were used. It has been shown that the full length intracellular domain of mouse Notch4 (N4ICwt) exhibits very weak transcriptional activity in vitro. When the TAD/PEST domains are deleted (N4IC), however, Notch4 can upregulate downstream reporters. For Notch1, however, full length Notch1 (N1ICwt) shows greater activation than Notch1 without the TAD/PEST domains (N1IC, See Figure 2). Therefore, a construct was made with the intracellular domain of Notch1 with the Notch4 TAD/PEST domain (N1/N4). The activity was monitored using the 4XCSL, Hes1, and Hes5 promoters. 4XCSL is an artificial construct, while the Hes promoters have been linked to proper mammalian lung, retinal and pancreatic differentiation, neurogenesis, and T-cell development. Using these promoters and comparing the different Notch constructs, we are attempting to determine whether the Notch4 TAD/PEST domain is transferable. If so, the activitiy profile of N1/N4 should resemble that of N4ICwt versus N1ICwt in the different assays.
    URI
    http://hdl.handle.net/10920/4349
    Collections
    • Diebold Symposium Posters and Schedules [444]

    Related items

    Showing items related by title, author, creator and subject.

    • Thumbnail

      Function of the TAD/PEST Domains in the Mouse Notchl and Notch 4 Genes 

      Flewelling, Daniel T. (Kalamazoo College, 2002)
      The Notch gene, cell-surface protein, is necessary for cell-fate decisions in many developing organisms. There are multiple homologs in mammals, including four Notch proteins in mice. Improper signaling has been linked ...
    • Thumbnail

      Downregulation of Notch Signaling Pathway Decreases Primary Cilium Lengths in Autosomal Dominant Polycystic Kidney Disease 

      Dong, Susan (Kalamazoo College, 2022-03-01)
      Autosomal dominant polycystic kidney disease (ADPKD) affects over half a million people and causes around 10% of end stage renal failure in the United States. One of the most prominent symptoms of ADPKD includes the presence ...

    Browse

    All of CACHECommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV
    Logo

    Kalamazoo College
    1200 Academy Street
    Kalamazoo Michigan 49006-3295
    USA
    Info 269-337-7000
    Admission 1-800-253-3602

    About K
    Academics
    Admission
    Alumni Relations
    Giving to K
    News & Events
    Student Life
    Sitemap
    Map & Directions
    Contacts
    Directories
    Nondiscrimination Policy
    Consumer Information
    Official disclaimer
    Search this site


    Academic Calendars
    Apply
    Bookstore
    Crisis Response
    Employment
    Library
    Registrar
    DSpace Express is a service operated by 
    Atmire NV