Transformation of Arabidopsis thaliana with CRISPR/Cas9 -modified AtHMA4 (Heavy-metal ATPase-4) using floral dip to increase Zn2+ tolerance
Abstract
This study utilizes recent plasmid-designing technology to
customize guide-RNA plasmids encoded with the
CRISPR/Cas9 system in order to artificially increase zinc
tolerance in Arabidopsis thaliana via the truncation of the Cterminal
peptide sequence of the AtHMA4 enzyme. A.
thaliana plants were transformed via floral dip in order to
grow future generations of plants in hopes of increasing their
nutritional value to combat global malnutrition.