Characterization of a Novel Prostaglandin Generating Factor from Anaphylaxed Human Lung
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A novel low molecular weight prostaglandin generating factor was isolated from the supernatant of anaphylaxed human lung. The factor was found to be sensitive to degradation by trypsin. The activity is characterized by rapid reaction kinetics, peaking in prostaglandin production five minutes after incubation initiation. The factor was found to exhibit activity throughout a range of physiologic pH’s and temperatures, with a significant temperature optimum at 37-41 C. A dependence for activity was demonstrated to exist for divalent cations, primarily calcium.