A Time-Course Study of Sprague-Dawley Bat Kidney Homogenates of the Renal Cortex, Junction and Medulla by Selected Tissue Assay Techniques
Larke, Daryl S.
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Three major assays were used in order to measure various parameter changes of the overall renal system. The first assay was a Lowry protein assay, which measured the amount of protein present in any given tissue sample. The second major assay used was to study the activity of the enzyme system catalyzed by the enzyme adenyl cyclase. The third, and most important, assay included the radioimmunoassay of the amounts of cAMP in the rat renal tissues of the cortex, junction and medulla. These studies would hopefully shed new light upon the amount of cAMP present by measurement of its quantitative levels through a time-course study of the hypertrophying kidney, coupled with the normal kidney, which could serve as the control.