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dc.contributor.advisorGoodson, Theodore III
dc.contributor.authorDandekar, Anuja B.
dc.descriptionvii, 18 p.en_US
dc.description.abstractThrough using the two-photon excited fluorescence method, the two-photon absorption cross section was measured in order to study the binding modes of different fluorescent drugs on RNA. Two photon absorption and time correlated single photon counting were used to examine the different components of the drugs. Based on the TPA cross-section trend it was determined which dyes are groove-binders and which are intercalators. The ones showing a downward trend are representative of intercalators and the ones showing an upward trend are groove-binders. Acridine orange and Hoechst 33258 were tested. Acridine Orange showed a downward trend indicating it is an intercalating dye while Hoechst 33258 had an upward trend representative of a groovebinding drug. Time correlated single photon counting experiments were conducted on both fluorophores as a quantitative analysis to determine if a qualitative experiment with HeLa cells would be effective.en_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Chemistry Senior Individualized Projects Collection
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleAnalysis of Drug Binding Modes with RNA using Two-Photon Excited Fluorescenceen_US

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  • Chemistry Senior Individualized Projects [860]
    This collection includes Senior Individualized Projects (SIP's) completed in the Chemistry Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

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