Analysis of Drug Binding Modes with RNA using Two-Photon Excited Fluorescence
Dandekar, Anuja B.
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Through using the two-photon excited fluorescence method, the two-photon absorption cross section was measured in order to study the binding modes of different fluorescent drugs on RNA. Two photon absorption and time correlated single photon counting were used to examine the different components of the drugs. Based on the TPA cross-section trend it was determined which dyes are groove-binders and which are intercalators. The ones showing a downward trend are representative of intercalators and the ones showing an upward trend are groove-binders. Acridine orange and Hoechst 33258 were tested. Acridine Orange showed a downward trend indicating it is an intercalating dye while Hoechst 33258 had an upward trend representative of a groovebinding drug. Time correlated single photon counting experiments were conducted on both fluorophores as a quantitative analysis to determine if a qualitative experiment with HeLa cells would be effective.