Show simple item record

dc.contributor.advisorFurchak, Jennifer R.W.
dc.contributor.authorWilkey, Clayton J.
dc.date.accessioned2019-07-08T14:15:14Z
dc.date.available2019-07-08T14:15:14Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10920/37203
dc.descriptionvi, 20 p.en_US
dc.description.abstractAllosterically regulated RNA aptamers have been observed in nature and have recently been exploited to target specific molecules of interest based on in vitro selection. RNA aptazymes have been developed as a detection method for small molecules that could potentially replace the use of antibodies, but a roadblock that has yet to be overcome is the straightforward implementation of the aptazymes into cellular context. This study optimizes the DFHBI-1T binding RNA aptamer “Broccoli” paired with the 5-HTP scaffolded guanine riboswitch (GR) via a communication module (CM). Varying ion concentrations were investigated at both 25°C and 37°C (for pseudo-cellular context) to determine if the 5GR-II aptamer could be implemented into a cellular environment. The data suggest that the Broccoli aptamer is K+ dependent to bind to DFHBI-1T in the absence of sufficient 5-HTP. Optimal fold induction and KD occurred with the 5GR-II (U5) aptamer in standard ion conditions (Mg2+ [10 mM], Na+ [250 mM], and K+ [50 mM]) at 37°C.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherKalamazoo Collegeen_US
dc.relation.ispartofKalamazoo College Chemistry Senior Individualized Projects Collection
dc.rightsU.S. copyright laws protect this material. Commercial use or distribution of this material is not permitted without prior written permission of the copyright holder. All rights reserved.
dc.titleOptimization of the Allosterically Regulated RNA Aptamer 5GR-II for in vivo Implementation Using Fluorescence Detectionen_US
dc.typeThesisen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

  • Chemistry Senior Individualized Projects [860]
    This collection includes Senior Individualized Projects (SIP's) completed in the Chemistry Department. Abstracts are generally available to the public, but PDF files are available only to current Kalamazoo College students, faculty, and staff.

Show simple item record