Synthesis and Purification of Nanoparticles for Theoretical Optimal Biocompatibility in Future Cancer Therapy
Moote, Madison N.
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Cancer, a leading cause of death in today’s world, threatens to kill and destroy more and more lives each year. With very limited options for prevention, treatment after diagnosis is a limited path for most patients, requires incredible amounts of money, and is both, physically and emotionally, taxing for all of those involved. The immersion of nanotechnology with the applications to the newest development of the field nanomedicine brings hope to a disease that generally only brings depression. This innovative technology has biological properties and functionality that prove to be one of the least invasive and toxic forms of cancer treatment. In this experiment, preliminary tests to the F3 targeted nanoparticle, human serum albumin crosslinked with polyacrylamide and cysteine terminated. The results from the Bradford assay proved that the blank nanoparticle was effectively synthesized and Coomassie blue dye has a high affinity to bind to this nanoparticle. The results from DLS proved that the nanoparticle synthesized falls within the acceptable size range for nanoparticle therapy agents. Finally, the results from the MTT assay highlighted that the nanoparticles are biocompatible and nontoxic as a precursor. Combining these results shows that this nanoparticle platform is highly biocompatible and with further modifications could be a highly effective therapeutic agent.